Detection of starch granules in a living plant by optical second harmonic microscopy

G Mizutani, Y Sonoda, H Sano, M Sakamoto… - Journal of …, 2000 - Elsevier
We demonstrate a new method for detecting starch granules in a plant, based on optical
second harmonic microscopy. In this method a plant is irradiated with infrared laser pulses …

Selective observation of starch in a water plant using optical sum-frequency microscopy

Y Miyauchi, H Sano, G Mirzutani - JOSA A, 2006 - opg.optica.org
The photosynthesis, transfer, and storage of starch are the most important biogenic
processes occurring in plants. In order to observe the colorless and transparent starch …

Polarimetric second harmonic generation microscopy: An analytical tool for starch bioengineering

R Cisek, D Tokarz, L Kontenis, V Barzda… - Starch …, 2018 - Wiley Online Library
Second harmonic generation (SHG) is a nonlinear optical process that inherently generates
signal in non‐centrosymmetric materials, such as starch granules, and therefore can be …

Second-harmonic imaging of plant polysaccharides

G Cox, N Moreno, J Feijó - Journal of Biomedical Optics, 2005 - spiedigitallibrary.org
The application of second-harmonic generation (SHG) microscopy to plant materials has
been neglected hitherto even though it would seem to have promise for identification and …

Estimating the helical pitch angle of amylopectin in starch using polarization second harmonic generation microscopy

S Psilodimitrakopoulos, I Amat-Roldan… - Journal of …, 2010 - iopscience.iop.org
Starch granules are among the brightest natural second harmonic generation (SHG)
converters. They basically consist of amylose and amylopectin molecules and the source of …

Three‐dimensional structural imaging of starch granules by second‐harmonic generation circular dichroism

GY Zhuo, H Lee, KJ HSU, MJ Huttunen… - Journal of …, 2014 - Wiley Online Library
Chirality is one of the most fundamental and essential structural properties of biological
molecules. Many important biological molecules including amino acids and polysaccharides …

Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

R Cisek, D Tokarz, M Steup, IJ Tetlow… - Biomedical optics …, 2015 - opg.optica.org
Second harmonic generation (SHG) microscopy is employed to study changes in crystalline
organization due to altered gene expression and hydration in barley starch granules. SHG …

Stokes vector based polarization resolved second harmonic microscopy of starch granules

N Mazumder, J Qiu, MR Foreman… - Biomedical Optics …, 2013 - opg.optica.org
We report on the measurement and analysis of the polarization state of second harmonic
signals generated by starch granules, using a four-channel photon counting based Stokes …

Effect of molecular organization on the image histograms of polarization SHG microscopy

S Psilodimitrakopoulos, I Amat-Roldan… - Biomedical optics …, 2012 - opg.optica.org
Based on its polarization dependency, second harmonic generation (PSHG) microscopy has
been proven capable to structurally characterize molecular architectures in different …

Second harmonic generation imaging–a new method for unraveling molecular information of starch

ZY Zhuo, CS Liao, CH Huang, JY Yu, YY Tzeng… - Journal of structural …, 2010 - Elsevier
We present a new method, second harmonic generation (SHG) imaging for the study of
starch structure. SHG imaging can provide the structural organization and molecular …