abstract The components of extracellular matrix (ECM) may substitute for feeder layers that
promote the selfrenewal pathways in embryonic stem cells. Surface modification of …

Inadequate cell numbers in culture is one of the hurdles currently delaying the application of
human embryonic stem cells (hESCs) for transplantation therapy. Nanofibrous scaffolds …

Two-dimensional vs three-dimensional culture conditions, such as the presence of
extracellular matrix components, could deeply influence the cell fate and properties. In this …

AIM: To devise a simplified and efficient method for long-term culture and maintenance of
embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem …

Objective Three-dimensional (3D) biomimetic nanofiber scaffolds have widespread ap-
plications in biomedical tissue engineering. They provide a suitable environment for cel-lular …

Developing an efficient culture system for controlled human pluripotent stem cell (hPSC)
differentiation into selected lineages is a major challenge in realizing stem cell-based …

Embryonic stem cells (ESCs) are useful resources for drug discovery, developmental
biology and disease studies. Cellular microenvironmental cues play critical roles in …

Human embryonic stem cells and induced pluripotent stem cells have great potential in
research and therapies. The current in vitro culture systems for human pluripotent stem cells …

Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential
for regenerative medicine and tissue engineering applications. In the present study …

Human embryonic stem cells (hESCs) are routinely cultured on fibroblast feeder layers or in
fibroblast-conditioned medium, which requires continued supply of feeder cells and poses …