Although metagenomics has been previously employed for pathogen discovery, its cost and
complexity have prevented its use as a practical front-line diagnostic for unknown infectious …

Background The introduction of benchtop sequencers has made adoption of whole genome
sequencing possible for a broader community of researchers than ever before. Concurrently …

Adaptive mutations that have contributed to the emergence of influenza A pandemic (H1N1)
2009 virus, which can replicate and transmit among humans, remain unknown. We …

Background The emergence and rapid spread of the 2009 H1N1 pandemic influenza A virus
(H1N1pdm) in humans highlights the importance of enhancing the capability of existing …

Metagenomic next-generation sequencing (mNGS) is increasingly used for the unbiased
detection of viruses, bacteria, fungi, and eukaryotic parasites in clinical samples. Whole …

Pandemic 2009 influenza A virus (A/H1N1/2009) has emerged globally. In this study, we
performed a comprehensive detection of potential pathogens by de novo sequencing using …

Background Effective influenza surveillance requires new methods capable of rapid and
inexpensive genomic analysis of evolving viral species for pandemic preparedness, to …

The upper respiratory tract microbiome has an important role in respiratory health. Influenza
A is a common viral infection that challenges that health, and a well-recognized sequela is …

In March 2009, cases of influenza-like illness in Mexico caused by a novel H1N1 virus
containing genes from swine, avian, and human influenza strains were reported. Within …

Background Influenza A virus causes annual epidemics in humans and is associated with
significant morbidity and mortality. Haemagglutinin (HA) and neuraminidase (NA) gene …