Negative autoregulation matches production and demand in synthetic transcriptional networks
We propose a negative feedback architecture that regulates activity of artificial genes, or
“genelets”, to meet their output downstream demand, achieving robustness with respect to …
“genelets”, to meet their output downstream demand, achieving robustness with respect to …
Noise propagation in synthetic gene circuits for metabolic control
Dynamic control of enzyme expression can be an effective strategy to engineer robust
metabolic pathways. It allows a synthetic pathway to self-regulate in response to changes in …
metabolic pathways. It allows a synthetic pathway to self-regulate in response to changes in …
Synthetic negative feedback circuits using engineered small RNAs
Negative feedback is known to enable biological and man-made systems to perform reliably
in the face of uncertainties and disturbances. To date, synthetic biological feedback circuits …
in the face of uncertainties and disturbances. To date, synthetic biological feedback circuits …
Expression optimization and synthetic gene networks in cell-free systems
Synthetic biology offers great promise to a variety of applications through the forward
engineering of biological function. Most efforts in this field have focused on employing living …
engineering of biological function. Most efforts in this field have focused on employing living …
RNA compensation: a positive feedback insulation strategy for RNA-based transcription networks
The lack of signaling modularity of biomolecular systems poses major challenges toward
engineering complex networks. Directional signaling between an upstream and a …
engineering complex networks. Directional signaling between an upstream and a …
Engineering a functional small RNA negative autoregulation network with model-guided design
RNA regulators are powerful components of the synthetic biology toolbox. Here, we expand
the repertoire of synthetic gene networks built from these regulators by constructing a …
the repertoire of synthetic gene networks built from these regulators by constructing a …
Computational design of synthetic regulatory networks from a genetic library to characterize the designability of dynamical behaviors
G Rodrigo, J Carrera, A Jaramillo - Nucleic acids research, 2011 - academic.oup.com
The engineering of synthetic gene networks has mostly relied on the assembly of few
characterized regulatory elements using rational design principles. It is of outmost …
characterized regulatory elements using rational design principles. It is of outmost …
Synthetic control systems for high performance gene expression in mammalian cells
G Lillacci, Y Benenson, M Khammash - Nucleic acids research, 2018 - academic.oup.com
Tunable induction of gene expression is an essential tool in biology and biotechnology. In
spite of that, current induction systems often exhibit unpredictable behavior and performance …
spite of that, current induction systems often exhibit unpredictable behavior and performance …
Mathematical modeling of RNA-based architectures for closed loop control of gene expression
Feedback allows biological systems to control gene expression precisely and reliably, even
in the presence of uncertainty, by sensing and processing environmental changes. Taking …
in the presence of uncertainty, by sensing and processing environmental changes. Taking …
Regulatory dynamics of synthetic gene networks with positive feedback
YT Maeda, M Sano - Journal of molecular biology, 2006 - Elsevier
Biological processes are governed by complex networks ranging from gene regulation to
signal transduction. Positive feedback is a key element in such networks. The regulation …
signal transduction. Positive feedback is a key element in such networks. The regulation …